Activity assessment in ΔIntron17 mice.

A-B. Representative actograms from male (A) and female (B) mice under two different lighting conditions: (1) 12 h of light (indicated by the yellow shading), 12 h of dark (L/D) and (2) constant dark (D/D). Actograms depict locomotor activity (wheel revolutions) across time with days stacked vertically and double plotted with the x axis spanning 2 days (48 hours). Grey squares indicate activity onsets. C. Activity profiles averaged over 5 consecutive days of L/D conditions across sex and genotype. D. Endogenous period across sex and genotype. Mean endogenous periods per group: male +/+, 23.61; male ΔIntron17/+, 23.67; male ΔIntron17/ΔIntron17, 23.44; female +/+, 23.63; female ΔIntron17/+, 23.68; and female ΔIntron17/ΔIntron17, 23.65. E. Phase shift across sex and genotype. F. Intradaily variability in the L/D phase across sex and genotype. G. Intradaily variability in the D/D phase across sex and genotype. H. Interdaily stability in the L/D phase across sex and genotype. I-J. Average activity in the most active 10 hours of the day in the L/D (I) and D/D (J) phase. K-L. Start time of the most active 10 hours of the day the L/D (K) and D/D (L) phase. M-N. Average activity in the least active 5 hours of the day in the L/D (M) and D/D (N) phase. O-P. Start time of the least active 5 hours of the day the L/D (O) and D/D (P) phase. The number of animals used in the test is the same as in S3 Fig. If groups were normally distributed (D’Agostino & Pearson test) and didn’t have significantly different variance (F test), Ordinary one-way ANOVA test with Tukey’s multiple comparison test was used. If samples were not equally distributed or had different variances, Kruskal-Wallis test with Dunn’s multiple comparison test was used. Data are represented as mean ± SEM in all graphs.

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