AcoSV40 and AcoSI-1 fibroblasts maintain morphological characteristics and fibroblast markers (vimentin and αSMA) of pAFs.

(a) Phase images of passage 1 pAFs, AcoSV40, and AcoSI-1 fibroblast demonstrate similar morphology; scale = 100um. (b) pAFs, AcoSV40, and AcoSI-1 fibroblasts express vimentin at similar levels, but αSMA is weekly present in AcoSV40 and absent in AcoSI-1 fibroblasts under standard culture conditions. We demonstrated that under serum starvation, AcoSV40 and AcoSI-1 can (c) upregulate αSMA and (d) co-localize it to stress fibers; scale = 100μm.