Abundant cyst wall proteins contained two CBM49s (Luke(2) lectin), two 8-Cys domains (Leo lectin), or one CAA domain (Jonah(1) lectin).

The Luke(2) lectin had an N-terminal signal peptide (purple) and two CBM49s separated by short Ser- and Pro-rich spacers (light blue). The N-terminal CBM49 contained four Trp residues (red Ws), three of which were conserved in a C-terminal CBM49 of a tomato cellulase (larger font) and three of which were conserved in a single CBM49 of Dictyostelium cellulose-binding protein (XP_629733) (underlined). In contrast, the C-terminal CBM49 had two conserved Trp residues. The Leo lectin had a signal peptide and two unique domains (dark blue) containing eight Cys residues each (red Cs). The Jonah(1) lectin had a signal peptide, a Thr-, Lys-, and Cys-rich domain (gray), and a single CAA domain (green). Peptides used to immunize rabbits are underlined. An abundant Luke(3) lectin with three CBM49s, Leo(TKH) lectin with a Thr-, Lys-, and His-rich spacer, and an abundant Jonah(3) lectin with three CAA domains are shown in S2 Fig.