Absorption spectrophotometric titration of 3,5,7-tri-O-methyl-quercetin (quercetin3’4’OH).
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posted on 2016-10-27, 17:42 authored by Sabri Ahmed Cherrak, Nassima Mokhtari-Soulimane, Farid Berroukeche, Bachir Bensenane, Angéline Cherbonnel, Hafida Merzouk, Mourad Elhabiri(A) Absorption spectra, (B) absorption electronic spectra, and (C) complex formation evolution as a function of the [FeNTA]0 of the FeNTA complex with quercetin3’4’OH. Solvent: CH3OH/H2O (80/20 by weight); pH = 7.4 (Hepes buffer); T = 25.0(2°C; l = 1 cm. [Quercetin 3’4’OH]0 = 2.89 × 10−5 M. (D) Electrospray mass spectra of quercetin3’4’OH ferric complex (noted LH2) in the presence of NTA. Solvent: CH3OH, capillary voltage = 4000 V. [L.FeNTA]tot = 5 × 10−5 M; Negative mode; Fragmentor = -100 V.
