Public Library of Science
Browse
- No file added yet -

AMPK and Ca2+/calmodulin (CaM)-dependent protein kinase kinases (CaMKK2) contribute to the ghrelin activation of NPY neurons.

Download (371.02 kB)
figure
posted on 2017-09-06, 17:41 authored by Hiroshi Hashiguchi, Zhenyu Sheng, Vanessa Routh, Volodymyr Gerzanich, J. Marc Simard, Joseph Bryan

(A) AICAR (400 μM), an AMPK activator, mimics the effect of ghrelin. (B) Compound-C (30 μM), an inhibitor of AMPK, completely blocks the effect of oligomycin. (C) STO-609 (1 μM), a CAMKK2 inhibitor, partially reverses the effect of ghrelin. Ghrelin was applied at 100 pM (red bars). Traces are mean values from 5–20 cells.

History

Usage metrics

    PLOS ONE

    Categories

    Keywords

    CNQXTTA-PNPY-GFP neuronsSUR 1 agonistNPY GFP-labeled neuronsTrpm 4 antagonistsTrpm 4 modulatorsTTXpresynaptic action potentialsPKAKir 6.2ECAMPKghrelin actionMPNPY neuronsSUR 1growth hormone secretagogue receptorSUR 1 antagonistConclusions NPY neuronsNPY neurons ObjectivesPLCSNX 482-sensitive R-type voltagemeasure ion channel activityIRResults Acylated ghrelin depolarizedGHS-RGABA

    Licence

    CC BY 4.0

    Exports

    RefWorks
    BibTeX
    Ref. manager
    Endnote
    DataCite
    NLM
    DC