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Coccidioides detection level reported as the number of reactions testing positive at CT < 40 with logarithmic amplification using the CocciEnv qPCR assay.

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posted on 2023-05-25, 18:00 authored by Robert Wagner, Liliam Montoya, Jennifer R. Head, Simon Campo, Justin Remais, John W. Taylor

Soil samples considered positive for Coccidioides are enumerated in the first two columns and include samples with 8/8 positive wells and samples with either 6/8 or 7/8 positive wells. Samples considered negative for Coccidioides are enumerated in the next four columns and include all samples with < 6 positive wells. Note: settled dust samples used a more liberal criterion for positivity whereby samples with either 4/4 or 4/3 positive wells were considered positive for Coccidioides and are enumerated in the first two columns and samples considered negative for Coccidioides include all samples with < 3 positive wells and are enumerated in the following three columns. Numbers in parentheses in column headings indicate the number of positive wells for each detection level. * indicates detection level derived from a single composite (transect) soil sample (total subsamples from transect in numerator) from a single qPCR assay. Soil samples from Kearney soil cores in the Negative (2–3) category tested positive in no more than two of eight wells and from only one of the two, replicate qPCR test plates.