posted on 2014-12-10, 02:44authored byRong-Lin Wang, David C. Bencic, Natàlia Garcia-Reyero, Edward J. Perkins, Daniel L. Villeneuve, Gerald T. Ankley, Adam D. Biales
The CVs were calculated for each probe by individual batches under the Experiment factor and averaged over all batches. Further averaging these CVs across the entire transcriptome yielded an overall CV of 0.056 for fathead minnow (PPR) and 0.051 for zebrafish (DRE). The total number of orthologous genes identified between Agilent 015064 and 019597 was 6617, represented by 9311 and 6950 unique probes respectively. The PPR probes mapped to their EST sequences and ESTs to NCBI databases by BLAST all had a minimum E-value of E-06.
Within-batch variation as measured by coefficient of variation (CV).