Summary of BHT inhibition and chemical epistasis data.

In the Spider, 10% serum, Lee's, and GlcNAc columns, “+” refers to statistically significant BHT inhibitory activity (P value of <0.05 compared to the DMSO control; see Figure 2), whereas “−” refers to no statistically significant BHT inhibitory activity (P value of >0.05 compared to the DMSO control). In the efg1-T206E, ADH1pr-CPH1, db-cAMP, ras1-G13V, and gpa2-Q355L columns, “+” refers to the constitutively active mutant or db-cAMP being epistatic to the added BHT inhibitor, whereas “−” refers to the added BHT inhibitor being epistatic to the constitutively active mutant or db-cAMP. NT, not tested.