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Schematic diagram of full length and truncated MPS-1.

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posted on 2014-10-27, 17:08 authored by Pan Li, Pan Shi, Chaohua Lai, Juan Li, Yuanyuan Zheng, Ying Xiong, Longhua Zhang, Changlin Tian

(A) Various MPS-1 constructs with MPS-1(1–256), MPS-1(74–256) and MPS-1(134–256) depicted from top to bottom. The black segment (residues 46 to 68) represents the predicted transmembrane helix; (B) Model of the MPS1 domains showing the two phosphorylation sites within the cytoplasmic C-terminal domain. (C) SDS-PAGE of the purified MPS-1 proteins. Lane 1: molecular weight marker; lanes 2-4: purified MPS-1(1–256), MPS-1(74–256) and MPS-1(134–256).

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