posted on 2015-07-10, 05:28authored byVirginia S. Lioy, Andrea Volante, Nora E. Soberón, Rudi Lurz, Silvia Ayora, Juan C. Alonso
aThe plasmid or the strain bearing the relevant promoter(s) and gene(s) are indicated between parentheses.
bThe molecules/CFU were estimated as described in Materials and methods. The estimated numbers of molecules/cell are denoted between parentheses.
cThe Phsp δ:gfp, Phsp δD60A:gfp and Phsp ω:yfp genes integrated as unique copies into the amy locus in B. subtilis are under the control of the LacI expression cassette (LacI repressor-Hyper-Spank promoter, Phsp) (see S1 Table). The BG947, BG1097 and BG1469 strains were grown in the presence of 10 μM IPTG. NA, not applicable.
Protein (ω:YFP)2 binds Pδ and represses transcription.