Figure 8.tif (885.11 kB)
Northern blot analyses of siRNAs derived from BaMV in N. benthamiana and A. thaliana.
dataset
posted on 2010-08-02, 13:24 authored by Kuan-Yu Lin, Chi-Ping Cheng, Bill Chia-Han Chang, Wei-Chi Wang, Ying-Wen Huang, Yun-Shien Lee, Hsien-Da Huang, Yau-Heiu Hsu, Na-Sheng LinDetection of BaMV siRNA to confirm the abundant regions in BaMV or satBaMV-co-inoculated N. benthamiana and A. thaliana by different probes as indicated. Total RNA of 25 µg from BaMV-inoculated or satBaMV-co-inoculated N. benthamiana or 45 µg from BaMV-inoculated or satBaMV-co-inoculated A. thaliana were loaded onto 19% acrylamide/7 M urea gel. The same blot was used for detection by different probes. All films were processed overnight, except A. thaliana probe with CP (+).
