Effect of normalization strategy on statistical parameters calculated for Nppb and Vcam1 expression using single reference genes.
Datasets usually provide raw data for analysis. This raw data often comes in spreadsheet form, but can be any collection of data, on which analysis can be performed.
Two-tailed Mann-Whitney U test was used for calculating p-values. The statistical power was calculated for a Student's t-test using statistical parameters of log2 transformed expression data. Sample size (n) is number per group needed to obtain a power of at least 0.8. Nppb: group 2 vs. group 3; Vcam1: group 1 vs. group 3.