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Contamination of commercial RT-PCR reagents with murine leukemia virus (MLV).1

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posted on 2013-02-20, 10:41 authored by HaoQiang Zheng, Hongwei Jia, Anupama Shankar, Walid Heneine, William M. Switzer
1

The reverse transcriptase (RT) enzymes and kits were tested by quantitative protease (pro), polymerase (pol), gag and/or nested pol RT-PCR assays and results shown are number of positive tests out of the total number of replicates. Percentages for replicate testing and copies/reaction (rxn) are in parentheses.

2

Mouse DNA contamination was detected using mitochondrial DNA (mtDNA) and intracisternal A particle (IAP) PCR qPCR assays.

3

ND, not done.

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