ATHB17Δ113 can bind both Class II and Class I DNA targets and ATHB17Δ113 containing V182A-Q185A-N186A mutation cannot bind Class II DNA target in in vitro assay (measured by Surface Plasmon Resonance (SPR).

Binding affinities and Kinetic constants of ATHB17Δ113 interacting with Class I and Class II type DNA, measured by Biacore 2000, globally fitted. SPR measurements with Biacore 2000 were at 25°C in HBS-EP, 100 ug/ml BSA (10 mM HEPES pH 7.4, 150 mM NaCl, 3 mM EDTA, 0.005% Tween-20,100 ug/ml BSA). Equilibrium dissociation constant KD = koff/kon.