Primers designed for the identification of yeast species.

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posted on 2025-04-24, 17:48 authored by Hira Ejaz, Muhammad Mushtaq, Shereen Khan, Nasir Azim, Abrar Hussain, Kaleemullah Kakar, Muhammad Zubair Khan, Ayisha Hafeez, Syed Moeezullah

Primers designed for the identification of yeast species.

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tertiary care hospital several independent factors remains rarely studied intensive care units including pcr amplification high mortality rates multiple antifungal agents proved highly effective nosocomial infections worldwide established protocol using effective infection control clinical staff due study also sought specific molecular markers including molecular diagnostics antifungal susceptibility tests prevalent among icu culture test revealed highlighting multidrug resistance yeast malt agar identified yeast species critical importance since candida auris resistant pathogen responsible limited laboratory settings yeast infections antifungal susceptibility limited settings molecular analyses candida auris using species targeted diagnostics species infection highly transmissible exhibits resistance detection due critical gap study represents study aimed novel multidrug limited awareness healthcare settings emerging multidrug tropicalis parapsilosis msp meyerozyma lusitaniae krusei kefyr guilliermondii glabrata famata dubliniensis albicans vitek 2 topoisomerase ii specific primers significant challenges prior research previously reported immunocompromised patients icu patients frequently misdiagnosed frequent misidentification first report earlier studies drug resistant dna sequencing diagnostic challenges dependent variable conduct inferential ccp ).amphotericin b advanced techniques additional species accuracy required 4 cultures 18s genes

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