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Optimum PNA-FISH fixation/permeabilization protocols predicted through RSM models for each species.

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posted on 2018-05-31, 17:34 authored by Rui Rocha, Carina Almeida, Nuno F. Azevedo

Fixation/permeabilization combinations included: paraformaldehyde and ethanol, paraformaldehyde and triton X-100 and paraformaldehyde and lysozyme. Negative control, predicted and average obtained fluorescence with error values (standard deviation) under optimum conditions are presented.

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