An A53T exposure affects the GSH/GSSG balance.
dataset
posted on 2016-09-13, 17:30 authored by Claire Hoenen, Audrey Gustin, Cindy Birck, Mélanie Kirchmeyer, Nicolas Beaume, Paul Felten, Luc Grandbarbe, Paul Heuschling, Tony HeurtauxPrimary microglial cells were treated for 6 h and 24 h with the A53T protein (5 μM) as well as with a reactive oxygen generator (menadione, 10 μM). After treatments, intracellular levels of reduced (GSH) and oxidized (GSSG) glutathione were measured. The ratio GSH/GSSG was established as a marker of oxidative stress. Results are given as mean ± SEM (n = 4 independent experiments).
