wtKLF6 knockdown and statin effects in lung epithelial cells.
(A) wtKLF6 mRNA expression was reduced by 47.2% compared to vector control (si-luc) cells (P = 0.008). (B) Expression of the wtKLF6 target genes ASAH1, CCL20, iNOS and PPARγ was examined by qRT-PCR in si-wtKLF6 cells compared to vector control. ASAH1, CCL20 and iNOS were inhibited in si-cells compared to vector control cells. PPARγ was unaffected in si-wtKLF6. (C) Simvastatin induced ASAH1 expression but independent of wtKLF6. Simvastatin induced CCL20 (D) and reduced iNOS (E) expression and these appeared to be mediated via wtKLF6. *P, ≤0.05, ** P, ≤0.01.