Figure_3.tif (292.57 kB)
wtKLF6 knockdown and statin effects in lung epithelial cells.
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posted on 2014-07-10, 02:59 authored by Emma Hennessy, Julie O'Callaghan, Marlies J. Mooij, Claire Legendre, Olga Camacho-Vanegas, Sandra C. Camacho, Claire Adams, John A. Martignetti, Fergal O'Gara(A) wtKLF6 mRNA expression was reduced by 47.2% compared to vector control (si-luc) cells (P = 0.008). (B) Expression of the wtKLF6 target genes ASAH1, CCL20, iNOS and PPARγ was examined by qRT-PCR in si-wtKLF6 cells compared to vector control. ASAH1, CCL20 and iNOS were inhibited in si-cells compared to vector control cells. PPARγ was unaffected in si-wtKLF6. (C) Simvastatin induced ASAH1 expression but independent of wtKLF6. Simvastatin induced CCL20 (D) and reduced iNOS (E) expression and these appeared to be mediated via wtKLF6. *P, ≤0.05, ** P, ≤0.01.
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cell biologyMolecular cell biologygeneticsgene expressionimmunologyImmune responseinflammationImmune systemInnate immune systemimmunityImmune activationmicrobiologybacteriologyGram negative bacteriaMedical microbiologyMicrobial pathogensBacterial pathogensInfectious diseasesBacterial diseasesPseudomonas infectionsPathology and laboratory medicinepathogenesisHost-pathogen interactionsknockdownstatinepithelial
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