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rpHsp90-induced proliferation of B-cell population.

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posted on 20.06.2011 by Mariana G. Corigliano, Andrea Maglioco, Melina Laguía Becher, Alejandra Goldman, Valentina Martín, Sergio O. Angel, Marina Clemente

(A) Fractionation of spleen cells. Whole spleen cells from BALB/c mice were incubated with (PE)-conjugated anti-mouse CD19 and FITC–conjugated anti-mouse CD3. CD19+ and CD3+ populations were fractionated by a positive sorting. (B) 2.5×105 whole spleen cells and 1×105 fractionated CD19+ cells were cultured for 3 days with or without rAtHsp81.2 or rNbHsp90.3. Polymyxin B (PX) was used at 2 µg/ml. Values represent the mean and standard deviations of triplicate samples and are representative of two experiments. Statistical analysis was performed by one-way analysis of variance (ANOVA) using the Bonferroni's Multiple Comparison Test. The asterisks indicate the statistically significant differences between the different stimuli and medium. **p<0.01, ***p<0.001.

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