pvs25 transcript numbers versus light microscopically determined P. vivax gametocyte counts.

Dilution series were made before storing samples in RNAprotect (5 dilution steps) or after RNA extraction of samples stored in TRIzol (3 dilution steps). Dots represent means of technical triplicates (TRIzol) or quintuplicates (RNAprotect) of dilutions except for the highest concentration (no replicates). Shaded grey shows the CI95 of the intercept of the regression of compiled sample sets.