mRNA reduction of the crystallins in miak mice at embryonic stages.

The relative levels of Cryaa, Cryab, Cryb1 and Cryga mRNA in the eye of wild-type (+/+) and miak/miak mice at E11.5, E12.5 and E14.5. The mRNA expression levels were measured by real-time RT-PCR analysis using specific primer sets (Table S1) for each gene. The values shown in each graph indicate the mean relative expression levels and the SDs of triplicate eye mRNAs. The expression levels in wild-type mice at E11.5 were assigned an arbitrary value of 1 for comparative purposes. *P<0.05; **P<0.01; ***P<0.001.