mRNA expression of CgACE.

A: Relative CgACE mRNA expression levels in adult tissues (upper left panel), development stages (upper right) and gametogenesis (bottom). The expression level and reference genes are indicated. The bottom diagram represents the sexual maturation. ***: p<0.001, two-way ANOVA followed by Bonferroni's post hoc test. B: CgACE in situ hybridization in adult oysters. S, sense riboprobe; AS, antisense riboprobe. The magnification (100×, 400×) and the sexual maturation stage (I, II and III) are indicated; g.t., gonadic tubule; r.t. , reserve tissue ; d.l., digestive lumen. Arrowheads indicate typical signal localization.