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mdt-15(tm2182) mutants have a zinc storage defect.

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posted on 2019-12-09, 18:31 authored by Naomi Shomer, Alexandre Zacharie Kadhim, Jennifer Margaret Grants, Xuanjin Cheng, Deema Alhusari, Forum Bhanshali, Amy Fong-Yuk Poon, Michelle Ying Ya Lee, Anik Muhuri, Jung In Park, James Shih, Dongyeop Lee, Seung-Jae V. Lee, Francis Christopher Lynn, Stefan Taubert

[A] Representative fluorescence and DIC micrographs of worms stained with FluoZin-3, showing zinc accumulation in the gut granules of wild-type (WT), mdt-15(tm2182), and hizr-1(am286) worms grown without zinc supplementation. [B-C] Quantification of the number of gut granules in wild-type (WT), mdt-15(tm2182), and hizr-1(am286) worms; every dot represents an individual worm. Error bars: standard deviation; n = 10 for WT in [B], 11 for mdt-15(tm2182); 15 for WT in [C]; 14 for hizr-1(am286) (totals from 3 independent repeats for [B] and 4 for [C]). Statistical analysis: * p < 0.05, n.s. = not significant, unpaired Student’s t-test, comparisons to WT. [D] Representative fluorescence and DIC micrographs of worms stained with FluoZin-3, showing zinc accumulation in the gut granules of wild-type (WT), mdt-15(tm2182), cdk-8(tm1238), and hizr-1(am286) worms grown with 200μM zinc supplementation. [E] Quantification of the number of gut granules in wild-type (WT), mdt-15(tm2182), and cdk-8(tm1238) worms; every dot represents an individual worm. Error bars: standard deviation; n = 10 for WT, 13 for mdt-15(tm2182), 11 for cdk-8(tm1238) (totals from 3 independent repeats). Statistical analysis: **** p < 0.0001, One-way ANOVA, multiple comparisons, Dunnett correction, compared to “WT”. [F] Quantification of the number of gut granules in WT and hizr-1(am286) worms; every dot represents an individual worm. Error bars: standard deviation; n = 13 for WT, 15 for hizr-1(am286) (totals from 4 independent repeats). Statistical analysis: *** p < 0.001, unpaired Student’s t-test, compared to “WT”. [G] Representative fluorescence and DIC micrographs of worms fed control (empty vector L4440), mdt-15, or fat-6 RNAi and stained with FluoZin-3, showing zinc accumulation in the gut granules of worms grown with 200mM zinc supplementation.[H] Quantification of the number of gut granules in worms fed control, mdt-15, or fat-6 RNAi; every dot represents an individual worm. Error bars: standard deviation. n = 6 for control (2 independent repeats), 3 for mdt-15 (1 repeat), and 5 for fat-6 (2 independent repeats). Statistical analysis: * p < 0.05, n.s. = not significant, One-way ANOVA, multiple comparisons, Dunnett correction, compared to control RNAi.

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