lincRNA-IBIN (CR44404) expression is strongly induced by Gram-positive and Gram-negative bacteria and its expression is regulated by the Toll and Imd pathways and a functional BAP complex.

A) In a whole transcriptome analysis, 28 genes were more than 18-fold upregulated after a M. luteus infection. The highest upregulation in infected flies was found in a long non-coding RNA gene, CR44404 (lincRNA-IBIN). p-value <0.005 in all selected genes (S1 Table). B) 16 upregulated lncRNA genes have more than a threefold expression change in response to a M. luteus infection in adult flies. p-value <0.05 in all selected lncRNA genes (S2 Table). C) lincRNA-IBIN expression is induced in Drosophila adults within two hours of an infection by M. luteus or E. cloacae. For fold-induction values, expression values in uninfected samples were set to 1. D) M. luteus-induced lincRNA-IBIN expression is dependent on the Toll pathway (the Toll pathway adaptor protein MyD88) function. E) E. cloacae-induced lincRNA-IBIN expression is dependent on the Imd pathway (Relish) function. In D and E, for fold-induction values, expression values in uninfected w, MyD88IR samples were set to 1 F) lincRNA-IBIN expression is induced in Drosophila adults upon silencing of the Drosophila inhibitor of κB factor cactus. G) lincRNA-IBIN expression is induced in Drosophila larvae with the constitutively active form of the Toll receptor, Toll10b. In F and G, for fold-induction values, expression values in uninfected/untreated w samples were set to 1. H) lincRNA-IBIN expression is also modestly induced by the ubiquitous overexpression of Imd with daughterless-GAL4 (da>Imd) in Drosophila larvae. For fold-induction values, the expression value of w,da> was set to 1. I-J) Both M. luteus and E. cloacae-induced lincRNA-IBIN expression is dependent on the functional chromatin remodeling BAP complex. In I and J, for fold-induction values, expression values in uninfected w, osaIR samples were set to 1.