<i>lincRNA-IBIN</i> expression is induced in immunogenic tissues and its cellular localization is mainly nuclear.

<p><b>A-B)</b><i>lincRNA-IBIN</i> is induced in the larval fat body and hemocytes after a <i>L</i>. <i>boulardi</i> infection and is dependent on the expression of the BAP complex member <i>osa</i> in these tissues. For fold-induction values, expression values in uninfected <i>w</i>, <i>osa</i><sup><i>IR</i></sup> samples were set to 1. <b>C)</b> qPCR for hemocyte-specific <i>Hml</i> (i) and fat body-specific <i>Lsp1α</i> (ii) was carried out to confirm the purity of the tissue fractions. <i>lincRNA-IBIN</i> was not found in the plasma fraction in large quantities (iii). <b>D)</b> RNA FISH performed in larval hemocytes shows that <i>lincRNA-IBIN</i> is mainly located in the nucleus; pink labelling (<i>lincRNA-IBIN</i>) co-localizes with blue nuclear labelling (DAPI). <b>i)</b> Negative control (without <i>lincRNA-IBIN</i> probes), <b>ii)</b> hemocytes from <i>w</i><sup><i>1118</i></sup> larvae showing the basal expression level and localization of <i>lincRNA-IBIN</i>, <b>iii)</b> hemocytes from larvae overexpressing <i>lincRNA-IBIN</i><sup><i>1</i></sup> (<i>HH>lincRNA-IBIN</i><sup><i>1</i></sup>) and infected with <i>L</i>. <i>boulardi</i>.</p>