gap40 KOi parasites collapse after initiation of the cell cycle.

Parasites were transiently transfected with imc1-tomato, allowed to express overnight before the addition of 50 nM rapamycin to induce excision of the gene. 4 hours post excision, imaging was initiated and images taken every 7 minutes, every second frame is displayed. The IMC1-expressing vacuole initiated endodyogeny at 162 min after imaging was initiated as indicated by the formation of daughter cell buds (arrows) and at the same point YFP expression was observed. Soon after the initiation of endodyogeny, the vacuole was observed to lose organization. Scale bar 5 μm.