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Por mRNA transcript and POR protein abundance under moderate diurnal light (200L/D) transitioning to constant illumination (200L/L).

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posted on 2016-07-01, 17:46 authored by Heather M. Hunsperger, Christopher J. Ford, James S. Miller, Rose Ann Cattolico

(a) Fold-change in por1 and por2 mRNA transcript abundance measured by RT-qPCR. (b) POR1 density per μg protein measured by Western blotting (bars; error bars show standard deviation) and normalized to cellular protein levels to attain POR1 density per cell (area chart). Representative blot data are shown below each sampled time point. (c) As in (b) but for POR2.

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    POR 1 protein abundance12 h lightPOR 2 abundancePOR 1diatom Phaeodactylum tricornutumPOR 2 protein levelsPOR protein abundancepor 1 mRNADuplicate Light-Dependent Protochlorophyllide Oxidoreductasespor 2 mRNApor 1 transcript abundancePOR 2 protein abundancespor 2. Little concordancePOR 2 protein abundancelight-dependent protochlorophyllide oxidoreductasesDiatom Phaeodactylum tricornutum Background Diatomspor 2 mRNA levelsdiel por 2 mRNA responsepor 1 mRNA abundancepor gene expression

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