P. gingivalis gingipains degrade JAM1 in IHGE cells.
(A) IHGE cells were infected for 1 h with P. gingivalis WT or the Δkgp ΔrgpA ΔrgpB mutant at an MOI of 100. The cells were then analyzed by immunoblotting with the indicated antibodies. (B–E) IHGE cells were transiently transfected with plasmid encoding Myc-mCherry-CLDN1 (B), Myc-mCherry-CLDN4 (C), Myc-mCherry-OCLN (D), or HA-TJP1 (E). After 48 h of incubation, cells were infected with P. gingivalis at an MOI of 100 for 1 or 3 h. The cells were then analyzed by immunoblotting with the indicated antibodies. β-actin was used as a loading control. IB, immunoblot.