pone.0219313.g001.tif (914.95 kB)
In vitro characterisation of produced and purchased proteins.
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posted on 2019-07-26, 17:25 authored by Anja Sophie Schmid, Dario NeriCartoon, SDS-PAGE gel and SEC profile of (A) the noncovalent dimer F8IL15Rα in diabody format (predicted monomer molecular mass 44.4 kDa), (B) muIL15RαFc (predicted monomer molecular mass 44.9 kDa, manufacturer information: 80–90 kDa, 42 kDa and 35 kDa in reducing conditions), (C) huIL15RαFc (predicted monomer molecular mass 45 kDa, manufacturer information: 75–90 kDa in reducing conditions), (D) muIL15 biolegend (13.3 kDa), (E) muIL15 peprotech (13.3 kDa), (F) huIL15 (12.9 kDa) and (G) aIL15mAb. CHO = Chinese hamster ovary, mu = mouse, F8Dd = F8 diabody, hu = human, Fc = crystallisable fragment, m = marker, IgG = immunoglobulin G, nr = nonreducing conditions and red = reducing conditions.
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SPRsize exclusion chromatographysurface plasmon resonanceIL 15-based biopharmaceuticalsIL 15R subunitsbind IL 15.mouse IL 15Rmouse IL 15F 8ILIL 15EDAcancer therapy applicationsSECIL 15 complexationcell-based activity assay noneIL 15R fusionsnovel interleukin -15 receptorinterleukin -15 complexation Interleukin -15F 8 antibodyfusion protein F 8ILIL 15R fusion proteinsfusion protein
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