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In situ IFN-γ and IL-10 expression in lymphocutaneous and fixed lesions of sporothrichosis.

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posted on 2016-09-13, 17:32 authored by Fernanda N. Morgado, Armando O. Schubach, Maria Inês Pimentel, Marcelo R. Lyra, Érica C. F. Vasconcellos, Claudia M. Valete-Rosalino, Fátima Conceição-Silva

The in situ IFN-γ and IL-10 expression was detected by immunohistochemistry. The arrows point positive areas (red/AEC– 3-amino-9-ethylcarbazole). The intensity of staining was scored in ten microscopic fields (200x magnification) as rare (at least 1 positive area / field), discrete (2–3 positive areas / field), moderate (4–5 positive areas / field) and intense (>5 positive areas / field). Scale bar = 10μm.

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