IRG1 expression in human immune cells under pro-inflammatory conditions.

RNA was extracted from (A) PBMCs-derived monocytes, (B) PBMCs-derived macrophages and (C) PBMCs-derived dendritic cells 6 hours after treatment with LPS (10μg/ml), TNFα (50ng/ml), IFNγ (10ng/ml) and LPS together with TNFα or IFNγ in independent donors (D1-D6). The bars show the mean of 3 technical replicates (± SEM) of IRG1 mRNA levels measured by real-time PCR normalised with L27 as the housekeeping gene. ***p < 0.001, **p < 0.01, *p < 0.05.