Fig 2.tif (976.33 kB)
IRG1 expression in human immune cells under pro-inflammatory conditions.
figure
posted on 2016-02-12, 07:56 authored by Aravind Tallam, Thaneer M. Perumal, Paul M. Antony, Christian Jäger, Joëlle V. Fritz, Laurent Vallar, Rudi Balling, Antonio del Sol, Alessandro MichelucciRNA was extracted from (A) PBMCs-derived monocytes, (B) PBMCs-derived macrophages and (C) PBMCs-derived dendritic cells 6 hours after treatment with LPS (10μg/ml), TNFα (50ng/ml), IFNγ (10ng/ml) and LPS together with TNFα or IFNγ in independent donors (D1-D6). The bars show the mean of 3 technical replicates (± SEM) of IRG1 mRNA levels measured by real-time PCR normalised with L27 as the housekeeping gene. ***p < 0.001, **p < 0.01, *p < 0.05.
