ppat.1008074.g002.tif (145.38 kB)

Ex vivo differentiation drives HIV-1 reactivation in a reproducible manner.

figure

posted on 2019-10-14, 17:27 authored by Elizabeth R. Wonderlich, Krupa Subramanian, Bryan Cox, Ann Wiegand, Carol Lackman-Smith, Michael J. Bale, Mars Stone, Rebecca Hoh, Mary F. Kearney, Frank Maldarelli, Steven G. Deeks, Michael P. Busch, Roger G. Ptak, Deanna A. Kulpa

a, Column plot showing the measured IUPM for QVOA (n = 7) and dQVOA (n = 3) performed on RAVEN participant 2147-R. Error bars are 95% confidence intervals generated by the program IUPMStats (http://silicianolab.johnshopkins.edu). %CV represents the coefficient of variation for each assay type. (p = 0.0004; unpaired t test with Welch’s correction). Black brackets indicate assays initiated on the same day and performed in parallel. %CV over each bracket represents the %CV for each batched assay set-up. b, Column plots showing the frequency of p24 positive wells after complete QVOA (left panel, white bars), differentiation alone as part of dQVOA (right panel, black bars), or after differentiation, activation, and outgrowth in complete dQVOA (right panel, white bars). For both panels, gray bars indicate the frequency of wells that remain p24 negative after completion of each assay. Frequency of HIV-1-Gag+ wells was determined using the top assay dilution for each assay.