Bacillus sp. JR3 esterase LipJ: A new mesophilic enzyme showing traces of a thermophilic past - Fig 6

Zymogram analysis [31,32] of cell extract and supernatant samples of cloned LipJ and Bacillus JR3, performed at 30°C (A) and 60°C (B). Lane 1: crude cell extract of E.coli expressing LipJ; Lane 2: supernatant of B. cereus 131, used for comparison; Lanes 3–4: soluble (3) and insoluble (4) cell extract of E.coli without the lipJ insert, used as a negative control; Lanes 5–6: concentrated supernatant (5) and crude cell extract (6) of Bacillus strain JR3.