gp120 downregulates Nef dependent IL-6 release.

ELISA of IL-6 concentration was measured in pg/ml in immDCs. The first column denotes untreated cells; the second column denotes cells treated with only gp120; the third column denotes cells treated with only Nef; the fourth column denotes cells treated with both gp120 and Nef; the fifth column denotes cells treated with heat denatured gp120 along with Nef. Data represent the mean±SEM (n = 3). Statistical analysis was performed using Student's t-test, with the levels of significance defined as p*<0.05 and p**<0.01.