<i>ept</i> mutations reduce levels of the Drosophila Rb ortholog Rbf1.

<p>Confocal images of larval eye discs containing clones of <i>H99</i> mutant cells (A–A″), <i>ept,H99</i> double-mutant cells (B–C″) or <i>ept,H99,psn</i> triple mutant cells (E–E″) marked by the absence of GFP (green) co-stained for CycE (red in A–B″), or Rbf1 (red in C–C″, and E–E″). Tracing in A″ and B″ outlines <i>H99</i> and <i>eptH99</i> mutant clones respectively. The anti-CycE signal was recorded at the same optical settings in panels A″ and B″. Arrowheads in panels B and B″ denote <i>ept,H99</i> double mutant clones that express CycE. (D–D″) MARCM-mediated expression of DN-DaPKC (blue) in <i>ept,H99</i> double-mutant cells marked by GFP (green) restores levels of Rbf1 (red). Arrows in panel E denote clones of <i>ept,H99,psn</i> cells that express normal levels of Rbf1 relative to adjacent control cells.</p>