Zymographic analysis of MMP-2 and MMP-9 activity after in vitro incubation with MVC.

Purified standard MMP-2 (0.35 ng) and MMP-9 (0.88 ng) were subjected to gelatin zymography as described in the text. After the electrophoresis, the gel was cut into lanes and each lane was incubated in the absence (control) or in the presence with MVC at the reported concentrations during the development of the zymograms. 1,10 phenantroline (4 mM) (PA) was used as a positive control. A: Staining and destaining of the gels revealed that inhibition of both MMP-2 and MMP-9 activity was observed only in the lane incubated in the presence of PA, but not in the lanes developed in the presence of MVC. B: Percentage of MMP-9 and MMP-2 inhibition of repeated analyses calculated in comparison to control.