YM155 increases the degradation of survivin, EGFR, and XIAP in PANC-1 cells.
A, After treatment of PANC-1 cells with YM155 for 24 hours, survivin, EGFR, and XIAP mRNA levels were determined using real-time RT-PCR. B, Survivin, EGFR, and XIAP levels in PANC-1 cells after treatment with 50 µg/ml cycloheximide (CHX) in the absence or presence of 100 nM YM155 for the indicated periods were examined by Western blotting. The ratio of survivin, EGFR, or XIAP∶actin expression compared with the control is shown for each lane. *, nonspecific.