Wet mounted erythrocytes subvitally stained with New Methylene Blue (NMB)

(A). A reticulocyte containing the dark reticular matter is indicated by an arrow. The chemical structure of NMB is inserted at the top right hand corner. Wet mounted erythrocytes subvitally stained with Giemsa (B). Two reticulocytes are indicated by arrows. The chemical structure of Giemsa is inserted at the top right hand corner. Wet mounts of Plasmodium vivax (trophozoite stage) infected erythrocytes subvitally stained with NMB (C) and Giemsa (D). The parasitized red cells are indicated by the arrows. The horizontal scale bar at the bottom right of each photomicrograph represents 10 µm. Linear Regression (E) and Bland-Altman (F) comparison of the percentage of reticulocytes (number of reticulocytes per 1000 erythrocytes) detected by Geimsa and NMB wet mount methodologies.