Vpr complementation does not affect Vpr-defective single-cycle HIV-1 infection of MDDCs.
(A) Vpr incorporation in VSV-G-pseudotyped, single-cycle HIV-Vpr+ and Vpr-complemented HIV-Vpr-. Virion pellets were analyzed by immunoblotting with anti-Vpr and anti-p24, respectively. (B) Vpr complementation does not affect single-cycle HIV-Vpr- infection of MDDCs. Infected cells were lysed at indicated times post infection for the detection of HIV-1 infection by measuring luciferase activity and normalized to protein content (20 µg/sample). cps, counts per second. The data shown represents one of three independent experiments carried out with three individual donors.