Values of parameters used in simulations.

Data were obtained either from our own measurements in MIO-M1 cells or from literature, as follows: i- Vcell and Ac in isotonic conditions were estimated from confocal images as previously reported [43]; ii- Pf was obtained from the measured cell volume changes during a hypoosmotic challenge using a modification of the Fick’s law [20]; iii- Vm corresponds to the value recorded using sharp electrode patch clamp technique by Limb et al [19]; iv- intracellular Na+, K+ and Cl concentration are the typical values for most cell types; v- extracellular Na+, K+ and Cl concentrations are the same as in experimental solutions; vi- ion permeabilities were chosen in order to obtain the Vm value of MIO-M1 cells; vii- the initial parameters presented for the Mannitol condition were obtained by simulating an extracellular solution change, from ISONaCl to ISOMannitol (see Materials and Methods section).