Figure_4.tif (1.84 MB)
V-ATPase, PPase activity assays and vacuolar pH measurements in OsVHA-A RNAi transgenic lines.
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posted on 2013-07-22, 02:52 authored by Huiying Zhang, Xiangli Niu, Jia Liu, Fangming Xiao, Shuqing Cao, Yongsheng Liu(A) Vacuolar H+-ATPase activity and (B) V-PPase activity were determined in wild type (WT) and three OsVHA-A RNA interference lines (OsV-5, OsV-11, and OsV-18). (C) The images showing emission intensities of vacuoles from epidermal root cells loaded with BCECF AM. Results shown are representative. Scale bars = 100 μM. (D) The vacuolar pH values calculated from (C). (E) V-ATPase proton-pumping measured by the quenching of ACMA fluorescence. Ten micrograms of tonoplast vesicles were applied to detect fluorescence density. Each bar represents three replications. Asterisks (*) indicate significant differences from WT at P<0.05.
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cropscerealsricedevelopmental biologyPlant growth and developmentecologyPlant ecologyPlant-environment interactionsgeneticsGene functionModel organismsPlant and algal modelsMolecular cell biologySignal transductionSignaling cascadesStress signaling cascadeCellular stress responsesPlant sciencePPaseassaysvacuolarphrnaitransgenic
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