VEGF repression effect on ovulation, pregnancy and implantation rate.
(A) Reversible repression system of VEGF gene expression. (B) Relative VEGF mRNA levels by qPCR (n = 5). (C, D) Protein expression by Western blot analysis and the quantification (n = 3). (E) Embryo collection at E3.5 (n = 45). (F) Embryo dissection at E9.5 (n = 11). (G) Embryo transfer experiment (n = 22). Dox+ represented mouse chow with 200 mg/kg doxycycline. Dox− represented regular mouse chow. Data are presented as mean ± SEM. *P<0.05, **P<0.005, ***P<0.001.