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Upregulation of HIF-1α/2α by vGPCR paracrine secretions.

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posted on 2011-04-29, 02:44 authored by Bruno C. Jham, Tao Ma, Jiadi Hu, Risa Chaisuparat, Eitan R. Friedman, Pier Paolo Pandolfi, Abraham Schneider, Akrit Sodhi, Silvia Montaner

(A) HMEC1s were exposed to media conditioned by control (Control CM) or vGPCR-expressing cells (vGPCR CM), in the presence of vehicle or Rapamycin (50 µM). mRNA levels of HIF-1α and HIF-2α and protein levels of HIF-1α, HIF-2α and VEGF are shown. (B) HMEC1s were transfected with increasing doses (20, 40 and 80 nM) of HIF-1β siRNA or Scrambled siRNA. Cells were then exposed to media conditioned by vGPCR-expressing cells. VEGF mRNA levels are shown. (C) Immunohistochemical analysis of HIF-1α and HIF-2α, and staining using an isotype-matched control antibody, in vGPCR tumor and human KS.

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