Ultrastructural analysis of chlamydial inclusions.

A) HeLa cells overexpressing GFP, GFP-Rab14wt, GFP-Rab14S25N and GFP-Rab14ΔGCGC infected with C. trachomatis serovar L2 (MOI 5) were fixed at 24 h p.i. and processed for TEM. The arrow head shows an EB (smaller and electron dense) and the large arrow shows a RB (larger and less electron dense). Bar 2.25 µm. B) GFP-Rab14ΔGCGC overexpressing cells infected with C. trachomatis serovar L2 (MOI 5) for 24 h. Left panel shows an inclusion containing enlarged abnormal chlamydial developmental forms. Right panel shows an inclusion containing membrane structures and ghost bacterial forms. Bar 2.25 µm. C) Pie graphs show the relative proportion of reticulate bodies (RBs), elementary bodies (EBs) and large aberrant bacteria (ABs) and ghost bacterial forms (GFs) inside inclusions from GFP, GFP-Rab14wt, GFP-Rab14S25N and GFP-Rab14ΔGCGC overexpressing cells infected with C. trachomatis serovar L2 (MOI 5) for 24 h analyzed by TEM. At least 20 cells of each condition from three independent experiments were quantified.