U0126 treatment reduces the dwelling of PDGFRA in recycling system.

A. Representative confocal images of PDGFRA and Rab11 staining in glioma tissues of sample #2. B. The same condition as in A, but EEA-1 was detected. C. Immunostaining of PDGFRA and Rab11 with or without U0126 treatment in cell line #2. Arrows indicate the colocalized clusters. D. Average of PDGFRA co-localization to Rab11 detected under conditions as in C (18 cells in each condition, ***p<0.001, t-test). E. Immunostaining of PDGFRA and EEA-1 before and after U0126 treatment in cell line #2. Arrows indicate the colocalized clusters. F. Average of PDGFRA co-localization to EEA-1 under the same conditions as in E (28 cells in each condition, **p<0.01, t-test). G. Same as in E but Giantin was detected. The three separated images (right), the top, middle and bottom indicating Giantin, PDGFRA and respectively, are the zoom-in from box in left picture. H. Average of PDGFRA co-localization to Giantin under the same conditions as in G (42 cells in each condition. **p<0.01, t-test).