Transient inhibition of Mapk3/1 reduces transcription and translation of transcription factor Egr1.

A) Treatment of immature mice with single dose of 25μg/g of PD0325901 inhibited the expression of hCG-induced expression of Egr1 1h and 4h hCG. B) Abundance of Egr1 protein in granulosa cells of the inhibitor and vehicle treated mice. Actb was used as loading control.