Figure_1.tif (3.21 MB)
Topotecan treatment triggers autophagy in colon cancer cells.
figure
posted on 2012-09-14, 01:45 authored by Dan-Dan Li, Ting Sun, Xiao-Qi Wu, Shu-Peng Chen, Rong Deng, Shan Jiang, Gong-Kan Feng, Jing-Xuan Pan, Xiao-Shi Zhang, Yi-Xin Zeng, Xiao-Feng ZhuA. HCT116, LS-174T and HT29 cells were transfected for 24 h with an expression construct encoding LC3 fused to the yellow fluorescent protein (YFP-LC3). Thereafter, the cells were treated with or without 1 µg/mL topotecan (TPT) for 24 h and visualised under a confocal microscope. B. The indicated cells were treated with the indicated concentrations of topotecan for 24 h. The lysates were analysed by immunoblotting with The LC3 and P62 antibodies.
