Figure_4.tif (5.61 MB)
Tomato expression in E18.5 Fgf10iCre/+; Tomatoflox/+ lungs.
figure
posted on 2012-06-13, 01:33 authored by Elie El Agha, Denise Al Alam, Gianni Carraro, BreAnne MacKenzie, Kerstin Goth, Stijn P. De Langhe, Robert Voswinckel, Mohammad K. Hajihosseini, Virender K. Rehan, Saverio BellusciRecombination was induced at E15.5 by a single IP injection of tamoxifen. Note the absence of Tomato expression in Fgf10+/+; Tomatoflox/+ lungs (A; n = 3). The regions in the dotted boxes are magnified in (B) and (C). Tomato-positive cells are observed in the lung mesenchyme and interlobular septae of Fgf10iCre/+; Tomatoflox/+ lungs (white arrows) (A’, B’; n = 3). Inset in B’ shows high magnification of interlobular septae. Labeled cells in the trachea arrange in ring-like structures (black arrows) (C’; n = 3). (A”-C”; n = 8) X-Gal staining of Fgf10LacZ/+ lungs at E18.5. Inset in B” shows high magnification of interlobular septae. Tam: tamoxifen.
