Time-response analysis showing percentage changes in 31P-NMR-detected metabolite levels following treatment of KNS42 pediatric glioblastoma cells with PI-103 (5×GI50).

Data are expressed as percentage of treated to control (% T/C) and presented as the mean ± SD, n≥3.

PE in KNS42 cells was not consistently detectable due to the low levels of this metabolite and was not affected by treatment with PI-103.

Glycerophosphoethanolamine (GPE) level was not affected by treatment with PI-103.

Two-tailed unpaired t test was used to compare results in treated cells to controls.

*P<0.05.