Figure_1.tif (927.24 kB)
Time-course of hypoxia on HIF1α, HIF2α and HIF3α protein nuclear level and mRNA expression in LNCaP, DU145 and PC3 cells.
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posted on 2014-05-06, 02:56 authored by Linda Ravenna, Lorenzo Principessa, Alessandra Verdina, Luisa Salvatori, Matteo Antonio Russo, Elisa PetrangeliCell were exposed to 1% O2 from 1 up to 48–72 h, according to the experimental design or left untreated. A Protein expression was detected in nuclear extracts by immunoblot analysis. A representative experiment for each gene in each studied cell lines is shown. β-actin was used as a loading control. B mRNA determinations were performed by real-time PCR, normalized to the housekeeping gene 18S rRNA and expressed as fold induction with respect to normoxic controls (set at 1). Mean values ± SE.
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BiochemistryOxidative damagecell biologyCell processesCellular stress responsesMolecular cell biologygeneticsgene expressionDNA transcriptionProtein translationimmunologyImmune responseinflammationClinical immunologyimmunityoncologyCancers and neoplasmsGenitourinary tract tumorsProstate cancerBasic cancer researchhypoxiamrnadu145pc3
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